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首页> 外文OA文献 >Macrophage Migration Inhibitory Factor Is Up-Regulated in Human First-Trimester Placenta Stimulated by Soluble Antigen of Toxoplasma gondii, Resulting in Increased Monocyte Adhesion on Villous Explants
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Macrophage Migration Inhibitory Factor Is Up-Regulated in Human First-Trimester Placenta Stimulated by Soluble Antigen of Toxoplasma gondii, Resulting in Increased Monocyte Adhesion on Villous Explants

机译：刚体弓形虫可溶性抗原刺激人类孕中期胎盘中的巨噬细胞迁移抑制因子上调，导致绒毛植株上单核细胞粘附增加。

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Considering the potential role of macrophage migration inhibitory factor (MIF) in the inflammation process in placenta when infected by pathogens, we investigated the production of this cytokine in chorionic villous explants obtained from human first-trimester placentas stimulated with soluble antigen from Toxoplasma gondii (STAg). Parallel cultures were performed with villous explants stimulated with STAg, interferon-γ (IFN-γ), or STAg plus IFN-γ. To assess the role of placental MIF on monocyte adhesiveness to human trophoblast, explants were co-cultured with human myelomonocytic THP-1 cells in the presence or absence of supernatant from cultures treated with STAg (SPN), SPN plus anti-MIF antibodies, or recombinant MIF. A significantly higher concentration of MIF was produced and secreted by villous explants treated with STAg or STAg plus IFN-γ after 24-hour culture. Addition of SPN or recombinant MIF was able to increase THP-1 adhesion, which was inhibited after treatment with anti-MIF antibodies. This phenomenon was associated with intercellular adhesion molecule expression by villous explants. Considering that the processes leading to vertical dissemination of T. gondii remain widely unknown, our results demonstrate that MIF production by human first-trimester placenta is up-regulated by parasite antigen and may play an essential role as an autocrine/paracrine mediator in placental infection by T. gondii.
机译：考虑到巨噬细胞迁移抑制因子（MIF）在被病原体感染时在胎盘炎症过程中的潜在作用，我们研究了在弓形虫可溶性抗原刺激下从人头三个月胎盘获得的绒毛状绒毛外植体中这种细胞因子的产生。 ）。平行培养是用STAg，干扰素-γ（IFN-γ）或STAg加IFN-γ刺激的绒毛外植体进行的。为了评估胎盘MIF对单核细胞与人滋养细胞粘附性的作用，在存在或不存在经STAg（SPN），SPN和抗MIF抗体处理的培养上清液中，将外植体与人骨髓单核细胞THP-1细胞共培养重组MIF。培养24小时后，用STAg或STAg加IFN-γ处理的绒毛外植体产生并分泌的MIF浓度明显升高。 SPN或重组MIF的添加能够增加THP-1的粘附性，在用抗MIF抗体治疗后这种粘附受到抑制。这种现象与绒毛外植体的细胞间粘附分子表达有关。考虑到导致刚地弓形虫垂直传播的过程仍然广为人知，我们的研究结果表明，人头三个月胎盘的MIF产生被寄生虫抗原上调，并且可能在胎盘感染中起自分泌/旁分泌介质的作用。由T. gondii。

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